Microbial rennet

Microbial rennet (Cheese rennet alternative)

Some molds such as Rhizomucor miehei are able to produce proteolytic enzymes. These molds are produced in a fermenter and then specially concentrated and purified to avoid contamination with unpleasant side products of the mold growth. At the present state of scientific research, governmental food safety organizations such as the EFSA deny QPS (Qualified Presumption of Safety) status to enzymes produced especially by these molds.

The flavor and taste of cheeses produced with microbial rennets tend towards some bitterness, especially after longer maturation periods.[1] These so-called "microbial rennets" are suitable for vegetarians, provided no animal-based alimentation was used during the production. (Aternative to cheese rennet )
history-of-rennet.

Vegetarian Cheeses

Vegetarian Cheeses

Vegetarian cheeses are made with rennets of non-animal origin. In the past, fig leaves, melon, wild thistle and safflower have all supplied plant rennets for cheese making. However, most widely available vegetarian cheeses are made using rennet produced by fermentation of the fungus Mucor miehei. Vegetarian cheese may also be made using a rennet from the bacteria Bacillus subtilis or Bacillus prodigiosum. Cheese rennet.

Advances in genetic engineering techniques mean that some vegetarian cheeses may now be made using chymosin produced by genetically engineered micro-organisms. The genetic material (DNA) which encodes for chymosin is introduced into a micro-organism which can then be cultured to produce commercial quantities of chymosin. This is done by extracting genetic material from calf stomach cells which acts as a template for producing the chymosin encoding DNA. This can then be introduced into the micro-organism. Once the genetic material is introduced there is no further need for calf cells. Alternatively, the chymosin encoding DNA can be bio-synthesised in the laboratory without the use of calf cells.

The chymosin produced is identical to that produced by calf stomach cells. The development of genetically engineered chymosin has been encouraged by shortages and fluctuations in cost of rennet from calves. It's manufacturers claim that genetically engineered chymosin will end the cheese making industry's reliance on the slaughter of calves.

Chymosin encoding DNA has been introduced into three different micro-organisms. These are the yeast Kluyveromyces lactis, the fungus Aspergillus niger var awamori, and a strain of the bacteria Escherichia coli. All of these have now been approved and cleared for use by the Ministry of Agriculture, Fisheries & Food. There is no legal requirement for manufacturers to state whether a genetically engineered rennet has been used in the cheese making process.

Vegetarian cheeses are widely available in supermarkets and health food stores. A wide variety of cheeses are now made with non-animal rennet and labelled as suitable for vegetarians. No particular type of cheese is exclusively vegetarian. Soft cheeses are as likely to be non-vegetarian as hard cheese.
Cheese rennet.

Types of Cheese

Types of Cheese
The type of cheese produced depends on the milk used and the cheese making process. The milk used may be full fat, semi-skimmed or fully skimmed, this affecting the fat content of the cheese. It may be pasteurised or unpasteurised. Milk from different animals and different breeds is important in determining the final flavour. As well as cow's milk, cheese may be made from sheep or goat's milk. Cheese rennet.

Soft cheeses may be fresh or ripened. Fresh cheeses include quark, cottage cheese and cream cheese. Ripened soft cheeses include Brie and Camembert. Semi-soft cheeses include Stilton, Wensleydale and Gorgonzola. Hard cheeses include Cheddar, Cheshire, and Gruyere. Parmesan is a strongly pressed, very hard, dry cheese ripened for 2-3 years and then grated. Whey cheeses such as Ricotta are made as a by-product of other cheeses from the whey removed during pressing. Processed cheeses are either made with trimmings that are left over from the manufacture of other cheeses, or from dried milk powder. Flavourings, colourings and other additives are used. (Cheese rennet)

Vegetable rennet

Vegetable rennet

Many plants have coagulating properties. Greeks and Romans sometimes used an extract of fig bark to coagulate milk, and in some parts of Greece this tradition continues.[citation needed] Other examples include nettles, thistles, mallow, and Creeping Charlie. Enzymes from thistle or cynara are used in some traditional cheese production in the Mediterranean.

These real vegetable rennets are also suitable for vegetarians. Vegetable rennet might be used in the production of kosher cheeses but nearly all kosher cheeses are produced with either microbial rennet or Genetically Modified rennet. Worldwide, there is no industrial production for vegetable rennet. Commercial so-called vegetable rennets usually contain rennet from the mold Mucor miehei.
Alternative to cheese rennet.

Modern Rennet

MODERN RENNET: Until 1990, cheese rennet was produced the old fashioned way (from abomasums), from various "vegetable" rennets (some of which, called microbial coagulant, are made from the microorganism Mucor miehei.) These days, at a cost one tenth of that before 1990, chymosin is produced by genetically engineered bacteria into which the gene for this enzyme has been inserted. When the bacteria are grown in large vats,they secrete rennin, and itis then purified for cheese making. Cheese Rennet is available commercially in tablet or in liquid form. You will find some cheese makers on the web who prefer liquid rennet and disparage the use of rennet tablets. Perhaps, if you are making hundreds of gallons of milk into cheese, buying bulk liquid would make sense, but for making one to ten gallons of milk into cheese, the tablets make sense.

History of Rennet

HISTORY OF CHEESE RENNET: Presumably, the first cheese was produced by accident when the ancients stored milk in a bag made from the stomach of a young goat, sheep or cow. They found that the day-old milk would curdle in the bag (stomach), yielding solid chunks (curds) and liquid (whey). Once they discovered that the curd-chunks could be separated out and dried, they had discovered a means by which milk, an extremely perishable food, could be preserved for later use. The addition of salt was found to preserve these dried curds for long periods of time.
At some point, someone discovered that the most active portion of the young animal's stomach to cause curdling was the abomasum, the last of the four chambers of the stomach of a ruminant animal. (In sequence, the four chambers are rumen, reticulum, omasum and abomasum.) In particular, the abomasum from a suckling kid or calf was especially active. The abomasum was cut it into strips, salted and dried. A small piece would be added to milk in order to turn it into curds and whey.

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